Dan BrantonDepartment of Molecular & Cellular Biology
Biological Laboratories 2004
16 Divinity Avenue, Cambridge, MA 02138
tel: (617) 495-2646; fax: (617) 384-7732
Dr. Branton is not taking new graduate students, at this time.
Nanopore SequencingA novel technology for sequencing individual DNA molecules using single-channel recording techniques has been conceived. Single molecules of DNA are drawn through a small channel or nanopore that functions as a sensitive detector. The detection schemes being developed will transduce the different chemical and physical properties of each base into a characteristic electronic signal. Nanopore sequencing has the potential of reading very long stretches of DNA at rates exceeding 1 base per millisecond.
For a more detailed look at this project, please go to http://mcb.harvard.edu/branton/nanopore.html.
Kasianowicz, J, E. Brandin, D. Branton, and D.W. Deamer. 1996. Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. U.S.A. 93: 13770-13773.
Branton, D. and J. Golovchenko. 1999. Adapting to nanoscale events. Nature 398: 660-661.
Akeson, M., D. Branton, J.J. Kasianowicz, E. Brandin, and D.W. Deamer. 1999. Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules. Biophys. J. 77: 3227-3233.
Meller, A., L. Nivon, E. Brandin, J. Golovchenko, and D. Branton. 2000. Rapid nanopore discrimination between single polynucleotide molecules. Proc. Natl. Acad. Sci. USA 97: 1079-1084.
Meller, A., L. Nivon, and D. Branton. 2001. Voltage-driven DNA translocations through a nanopore. Phys. Rev. Lett. 86: 3435-3438.
Li, J., D. Stein, C. McMullan, D. Branton, M.J. Aziz, and J.A. Golovchenko. 2001. Ion-beam sculpting at nanometre length scales. Nature 412: 166-169.
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